Polymerase Chain Reaction
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Test Yourself !
1. The three main steps in a PCR cycle are _____.
A. Denaturation, Annealing, Extension
B. Annealing, Primer synthesis, Recombination
C. Annealing, Denaturation, Extension
D. Polymerization, Chain elongation, Recombination
E. Extension, Annealing, Denaturation
2. At the beginning of each cycle, the temperature of the PCR reaction is raised for ____.
A. Attach the primer
B. Denature the double DNA strands
C. Renature the double DNA strands
D. Elongate the primer
E. Polymerize the DNA
3. For DNA amplification to occur, which of the following are needed?
A. loose ribonucleotides
B. DNA primers
C. Thermostable DNA polymerase
D. B and C
E. All of the above
4. Taq polymerase is a commonly used enzyme in PCR because this enzyme is ____.
A. a faster polymerase
B. thermostable
C. a primer replicase
D. thermolabile
E. not prone to errors
5. Taq polymerase is a thermostable enzyme isolated from ____.
A. Thermus aquaticus
B. Bacteriophage T4
C. Agrobacterium tumefaciens
D. Escherichia coli
E. Haemophilus influenza
6. Which exonuclease activity does Taq polymerase NOT have?
A. 5’ to 3’
B. 3’ to 5’
7.A primer is ____.
A. A short DNA / RNA molecule that acts as starting point of 3’ chain growth
B. A long DNA/ RNA sequence that elongated the template
C. A regulatory region of short distance from the 5’ end of a gene
D. An autonomously replicating piece of DNA that serves as a template
E. None of the above
8. Which end of the primer is extended by the Taq polymerase?
A. 5’
B. 3’
9. When DNA is heated, primers anneal to DNA strands.
A. True
B. False
10. Which of the following is an application of PCR technology?
A. Gene mapping
B. In vitro mutagenesis
C. Forensics
D. All of the above
11. For PCR, DNA can be isolated from the following source: ____.
A. preserved tissue
B. frozen tissue
C. spermatic tissue
D. fossil speciments
E. all of the above
12. In between Pfu polymerase and Taq polymerase, which has the lowest error rate?
A. Pfu
B. Taq
Check answers:
1. A
2. B
3. D
4. B
5. A
6. B
7. A
8. B
9. B
10. D
11. E
12. A