Basic of PCR

The Polymerase Chain Reaction (PCR) is a biochemical process involves in DNA replication in vitro. PCR is capable of amplifying segments of DNA from relatively small amount up to a billionfold in the test tube.

The thermocycles of PCR reaction are repeated until the desired PCR products are obtained. The strength of PCR is that the products resulting from one primer extension are the templates for the next cycle. Consequently, amount of target DNA is doubled after each cycle.

Principle of PCR

The thermocycles

How does PCR work at such HIGH temperatures?

Find out more!

Secret of enzyme used in PCR cycle.

Please read "PCR at High Temperature".

Have Fun!

PCR in a Test Tube! PCR is a technique used to amplify target DNA into millions and billions of DNA in test tube!
Automated pipettor will assist you during your research.
A researcher is carefully pipetting the samples before performing PCR.
Adding dye to the sample is easier for detection.
Gel Electrophoresis Samples are undergone gel electrophoresis to analyze based on sample size and to decode the DNA sequences after PCR cycle.
Double helix DNA Complex structure of DNA is first proposed by Watson and Crick in 1953.